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Article Dans Une Revue Physical Review E Année : 2018

Kinky DNA in solution: Small angle scattering study of a nucleosome positioning sequence

Résumé

DNA is a flexible molecule, but the degree of its flexibility is subject to debate. The commonly-accepted persistence length of lp ≈ 500Å is inconsistent with recent studies on short-chain DNA that show much greater flexibility but do not probe its origin. We have performed X-ray and neutron small-angle scattering on a short DNA sequence containing a strong nucleosome positioning element, and analyzed the results using a modified Kratky-Porod model to determine possible conformations. Our results support a hypothesis from Crick and Klug in 1975 that some DNA sequences in solution can have sharp kinks, potentially resolving the discrepancy. Our conclusions are supported by measurements on a radiation-damaged sample, where single-strand breaks lead to increased flexibility and by an analysis of data from another sequence, which does not have kinks, but where our method can detect a locally enhanced flexibility due to an AT-domain.
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Dates et versions

ensl-02433391 , version 1 (09-01-2020)

Identifiants

Citer

Torben Schindler, Adrián González, Ramachandran Boopathi, Marta Marty Roda, Lorena Romero-Santacreu, et al.. Kinky DNA in solution: Small angle scattering study of a nucleosome positioning sequence. Physical Review E , 2018, 98 (4), ⟨10.1103/PhysRevE.98.042417⟩. ⟨ensl-02433391⟩
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