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Article Dans Une Revue Journal of Infectious Diseases Année : 2020

A Role for Taok2 in Listeria monocytogenes Vacuolar Escape

Résumé

The bacterial pathogen Listeria monocytogenes invades host cells, ruptures the internalization vacuole, and reaches the cytosol for replication. A high-content small interfering RNA (siRNA) microscopy screen allowed us to identify epithelial cell factors involved in L. monocytogenes vacuolar rupture, including the serine/threonine kinase Taok2. Kinase activity inhibition using a specific drug validated a role for Taok2 in favoring L. monocytogenes cytoplasmic access. Furthermore, we showed that Taok2 recruitment to L. monocytogenes vacuoles requires the presence of pore-forming toxin listeriolysin O. Overall, our study identified the first set of host factors modulating L. monocytogenes vacuolar rupture and cytoplasmic access in epithelial cells.
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Dates et versions

hal-03009310 , version 1 (17-11-2020)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Juan Quereda, Camille Morel, Noelia Lopez-Montero, Jason Ziveri, Steven Rolland, et al.. A Role for Taok2 in Listeria monocytogenes Vacuolar Escape. Journal of Infectious Diseases, 2020, ⟨10.1093/infdis/jiaa367⟩. ⟨hal-03009310⟩
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